CGSC Strain#: 7974
Strain Designation: EP432     
Source of Strain: S. Schuldiner
Sex: F-
Chromosomal Markers: thr-1,
ΔnhaA1::kan,
Δ(lacY-lacZ)9998,
ΔnhaB3::Cm,
rpsL-(strR),
melB-(ts,LiD),
zjd-2334::Tn10Strain Comments: - This strain is sensitive to Na+ and should be grown on LBK medium(LB plus KCl (6.5 g/l) lacking NaCl).
- melB-(ts,LiD)-- allows growth on melibiose at 30 C, but not at 37 C; after several days at 37 C, spontaneous revertants will arise.
- ΔnhaB3::Cm was formerly called nhaB1
- = kanamycin resistant
- = tetracycline resistant
- LiD = lithium dependent
- strR = streptomycin resist.
- ts = Temperature Sensitive
References: - Pinner, E., Y. Kotler, E. Padan, S. Schuldiner 1993. Physiological role of nhaB, a specific Na+/H+ antiporter in Escherichia coli. J.Biol.Chem. 268:1729-1734