CGSC Strain#: 4941
Strain Designation: KG1673     
Source of Strain: T. Kawasaki
Sex: Hfr
Point of Origin#: 3
Map Position: 6.80
Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: relA1,
spoT1,
thi-33Strain Comments: - spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- thi-33-- was originally reported to confer Thi- phenotype and presumed to be gene for THI-P synthase, but Vander Horn et al. report THZ auxotrophy of thi-33 strains.
- thi-33 was formerly called thiB33
References: - Kawasaki, T, T Nakata, Y Nose 1968. Genetic mapping with a thiamine-requiring auxotroph of Escherichia coli K-12 defective in thiamine phosphate pyrophosphorylase. J. Bacteriol. 95(4):1483-5.