KG1673      Source of Strain:
Hfr Point of Origin#:
3 Map Position:
6.80 Direction of Marker Transfer:
Counterclockwise Chromosomal Markers: relA1
, thi-33Strain Comments:
- spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- thi-33-- was originally reported to confer Thi- phenotype and presumed to be gene for THI-P synthase, but Vander Horn et al. report THZ auxotrophy of thi-33 strains.
- thi-33 was formerly called thiB33
- Kawasaki, T, T Nakata, Y Nose 1968. Genetic mapping with a thiamine-requiring auxotroph of Escherichia coli K-12 defective in thiamine phosphate pyrophosphorylase. J. Bacteriol. 95(4):1483-5.