CA161      Source of Strain:
S. BrennerOther Designations:
Hfr Point of Origin#:
1 Map Position:
96.80 Direction of Marker Transfer:
Clockwise Chromosomal Markers: lacZ53
, thiE1Strain Comments:
- lacZ53(Am)-- is the Paris lac-Y14 amber.
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- e14--- : Loss of the e14 element makes the strain McrA-.
- spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- Signer, E., J.R. Beckwith, S. Brenner 1965. Mapping of suppressor loci in Escherichia coli. J.Mol.Biol. 14:153-166
- Brenner, S., J.R. Beckwith 1965. Ochre mutants, a new class of suppressible nonsense mutants. J.Mol.Biol. 13:629-637