CGSC Strain#: 4991
Strain Designation: CA275     
Source of Strain: S. Brenner
Sex: Hfr
Point of Origin#: 1
Map Position: 96.80
Direction of Marker Transfer: Clockwise
Chromosomal Markers: lacZ125(Am)
,
λ-,
e14-,
tyrT66(AS)
,
trpA49(Am)
,
relA1,
spoT1Strain Comments: - This is a LAM- derivative of CA265.
- lacZ125(Am)-- is the amber mutation from Zipser's strain NG125 and Brenner's CA274. It was formerly called lac125am by Brenner. In `interval 6' of Newton et al. JMB 14:290 (1965).
- e14--- : Loss of the e14 element makes the strain McrA-.
- trpA49(Am)-- is the amber mutation formerly called trp-8am by Brenner.
- spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- tyrT66(AS) was formerly called su+III
- tyrT66(AS) was formerly called supF66
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
References: - Brenner, S., J.R. Beckwith 1965. Ochre mutants, a new class of suppressible nonsense mutants. J.Mol.Biol. 13:629-637