CGSC Strain#: 5001
Strain Designation: CR34 Thy^-      Source of Strain: T. Okada
Sex: F- Chromosomal Markers: thr-1, leuB6(Am), fhuA21, cyn-101, lacY1, glnV44(AS), λ^-, rfbC1, thyA6, thiE1, deoC1
Strain Comments:

• From Meselson via Brenner called S0 by Brenner i.e. parent of "S" series.
• leuB6(Am)-- Suppressed by serU suppressor (supH)
• thyA6-- : Even with Luria or other complete media, supplement with thymine.
• thyA6-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• cyn-101 was formerly called cyn-1
• glnV44(AS) was formerly called supE44
• glnV44(AS) was formerly called su⁺_II
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor

References:

• Okada, T., K. Yanagisawa, F.J. Ryan 1960. Elective production of thymine-less mutants. Nature 188:340-341
• Okada, T., K. Yanagisawa, F.J. Ryan 1961. A method for securing thymineless mutants from strains of E. coli. Z.Vererbungsl. 92:403-412