CGSC Strain#: 4908
Strain Designation: E. coli 15 T-H-U-      Source of Strain: S.S. Cohen
Sex: F- Chromosomal Markers: his-67, thyA43, pyr-37
Strain Comments:

• This strain may also carry a mutation leading to a thymine low-requirement (deoB or deoC).
• his-67-- E. coli 15 mutation.
• thyA43-- E. coli 15 mutation.
• thyA43-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• pyr-37-- E. coli 15 mutation.

References:

• Cohen, SS, M Sekiguchi, JL Stern, HD Barner 1963. THE SYNTHESIS OF MESSENGER RNA WITHOUT PROTEIN SYNTHESIS IN NORMAL AND PHAGE-INFECTED THYMINELESS STRAINS OF ESCHERICHIA COLI. Proc. Natl. Acad. Sci. U.S.A. 49(5):699-707.