VS102      Source of Strain:
F- Chromosomal Markers: Δ(gpt-proA)62
, lacZp-topA76(L5F C662H)
, thiE1Strain Comments:
- This strain is CamR and KanR, with inducible topA and deleted topB; it requires IPTG at all temperatures and will grow at 30-42C on L + 15 ug/ml Clam and 1 mM IPTG.
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- lacZp-topA76(L5F C662H)-- replaces promoter topAp of allele topA78(G65N W79S substitutions in TopoI) with lacZp, resulting in an IPTG requirement for expression.
- lacZp-topA76(L5F C662H)-- : poor growth at 30C in absence of IPTG; At 42C, TopoI level is low and more negative supercoiling is observed, but the strain grows if it is topB+.
- zci-2234::cat-- is an insertion of cam at a unique SphI site in region 5' (counterclockwise) of the topA gene
- Δ(gpt-proA)62 was formerly called proA2
- galK2(Oc) was formerly called gal2
- glnV44(AS) was formerly called supE44
- glnV44(AS) was formerly called su+II
- rfbC1 was formerly called rfbD1
- thiE1 was formerly called thi-1
- = chloramphenicol resistant
- = kanamycin resistant
- AS = amber(UAG) suppressor
- L5F C662H = amino acid changes in TopoI
- Oc = ochre(UAA) mutation
- Stupina, V.A., J.C. Wang 2005. Viability of Escherichia coli topA mutants lacking DNA topoisomerase I. J.Biol.Chem. 280:355-360