CGSC Strain#: 4877
Strain Designation: DF1311      Source of Strain: D. Fraenkel
Sex: Hfr    Point of Origin#: 2A   Map Position: 12.20   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: fhuA22, pgl-1, ompF627(T2R), fadL701(T2R), relA1, pitA10, spoT1, rrnB-2, pgi-2, mcrB1, creC510
Strain Comments:

• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• rrnB-2-- : loss of spacer loop sequence
• pgi-2-- : pgi mutants utilize glucose by the hexose monoP shunt; strains with this allele grew on glucose at 1/3 parental rate. Slow growth on Gal,Mal,Lac;normal on Fru and gluconate.
• creC510-- The constitutive phenotype is due to an R77P amino acid substitution
• creC510 was formerly called phoM510
• Const = constitutive
• T2R = T2 Resistant

References:

• Kupor, SR, DG Fraenkel 1969. 6-phosphogluconolactonase mutants of Escherichia coli and a maltose blue gene. J. Bacteriol. 100(3):1296-301.