CGSC Strain#: 4862
Strain Designation: W4680      Source of Strain: J. Lederberg
Sex: F- Chromosomal Markers: thr-1, ΔlacZ39, glnX44(AS), rfbC1, rpsL45(strR) or rpsL110(strR), thiE1, melB4
Strain Comments:

• ΔlacZ39-- is a deletion of ca. 1/3 of the lacZ gene.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• melB4-- : Found to lack TMG permease II, Schmitt, 1968, J.Bacteriol. 96:462
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• AS = amber(UAG) suppressor
• strR = streptomycin resist.

References:

• Cook, A., J. Lederberg 1962. Recombination studies of lactose nonfermenting mutants of Escherichia coli K12. Genetics 47:1335-1353
• Ullmann, A, F Jacob, J Monod 1967. Characterization by in vitro complementation of a peptide corresponding to an operator-proximal segment of the beta-galactosidase structural gene of Escherichia coli. J. Mol. Biol. 24(2):339-43.
• Schmitt, R 1968. Analysis of melibiose mutants deficient in alpha-galactosidase and thiomethylgalactoside permease II in Escherichia coli K-12. J. Bacteriol. 96(2):462-71.