CGSC Strain#: 4035
Strain Designation: AB4035     
Source of Strain: M.G. Marinus
Sex: F'
Episome/Plasmid: F42
Plasmid Markers/Mutations: lac Chromosomal Markers: thr-1,
leuB6(Am)
,
fhuA21,
lacY1,
glnX44(AS)
,
λ-,
thyA6,
rpsL67(strR)
,
thiE1,
dnaB500(ts)
,
deoC1Strain Comments: - dnaB500 = DNATS-500 (Bonhoeffer)= tsm-1 (Marinus).
- leuB6(Am)-- Suppressed by serU suppressor (supH)
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- thyA6-- : Even with Luria or other complete media, supplement with thymine.
- thyA6-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
- dnaB500(ts) was formerly called tsm-1
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- strR = streptomycin resist.
- ts = Temperature Sensitive
References: - Marinus, MG, EA Adelberg 1970. Vegetative Replication and Transfer Replication of Deoxyribonucleic Acid in Temperature-Sensitive Mutants of Escherichia coli K-12. 104(3):1266-1272.