CGSC Strain#: 1877
Strain Designation: Lin 61      Source of Strain: E.C.C. Lin
Other Designations: AB1877
Sex: Hfr    Point of Origin#: 2A   Map Position: 12.20   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: garB10, fhuA22, lac-20, ompF627(T2R), relA1, pitA10, spoT1, glpK1, rrnB-2, mcrB9999, creC510
Strain Comments:

• This strain is glpK^- but produces cross-reacting material upon induction.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• glpK1-- : a stable, CRM⁺ allele. Grows on glycerol-P, not glycerol; induction by G-3-P, not glycerol
• rrnB-2-- : loss of spacer loop sequence
• creC510-- The constitutive phenotype is due to an R77P amino acid substitution
• creC510 was formerly called phoM510
• Const = constitutive
• T2R = T2 Resistant

References:

• Koch, J.P., H. Hayashi, E.C.C. Lin 1964. The control of dissimilation of glycerol and L-alpha-glycerophosphate in Escherichi coli. J.Biol.Chem. 239:3106-3108
• Hayashi, SI, EC Lin 1965. Product induction of glycerol kinase in Escherichia coli. J. Mol. Biol. 14(2):515-21.
• Cozzarelli, NR, EC Lin 1966. Chromosomal location of the structural gene for glycerol kinase in Escherichia coli. J. Bacteriol. 91(5):1763-6.