CGSC Strain#: 4347
Strain Designation: KL335      Source of Strain: M.C. Cappello
Sex: Hfr    Point of Origin#: 2A   Map Position: 12.20   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: lacZ608(Am), lacI22, λ^-
Strain Comments:

• lacZ608(Am)-- is an amber mutation (Newton et al. 1965 J. Mol. Biol. 14:290), aka lacZ^-NG608 by Zinder, in segment 10 of the lacZ gene (Zipser et al. 1970).
• lacI22 was formerly called lacI3
• lacI22 was formerly called lac_i3^-
• Am = amber(UAG) mutation

References:

• Birge, EA, KB Low 1974. Detection of transcribable recombination products following conjugation in rec+, reCB- and recC-strains of Escherichia coli K12. J. Mol. Biol. 83(4):447-57.