KL20      Source of Strain:
F+ Chromosomal Markers: λ-
, thiE1Strain Comments:
- This strain is a spontaneous F+ revertant of Hfr 3000.
- e14--- : Loss of the e14 element makes the strain McrA-.
- spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- thiE1 was formerly called thi-1
- Low, K.B. 1973. Rapid mapping of conditional and auxotrophic mutants of Escherichia coli K-12. J.Bacteriol. 113:798-812