W3CG      Source of Strain:
F- Chromosomal Markers: λ-
, rph-1Strain Comments:
- gapA10::Tn10-- -- Note that this strain should grow on minimal medium containing both glycerol and malate, but will not grow on minimal medium with glucose or rich media.
- IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
- rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
- = tetracycline resistant
- Ganter, C., A. Pluckthun 1990. Glycine to alanine substitutions in helices of glyceraldehyde-3-phosphate dehydrogenase: effects on stability. Biochemistry 29:9395-9402