CGSC Strain#: 8166
Strain Designation: W3CG     
Source of Strain: Doe
Sex: F-
Chromosomal Markers: λ-,
gapA10::Tn10,
IN(rrnD-rrnE)1,
rph-1Strain Comments: - gapA10::Tn10-- -- Note that this strain should grow on minimal medium containing both glycerol and malate, but will not grow on minimal medium with glucose or rich media.
- IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
- rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
- = tetracycline resistant
References: - Ganter, C., A. Pluckthun 1990. Glycine to alanine substitutions in helices of glyceraldehyde-3-phosphate dehydrogenase: effects on stability. Biochemistry 29:9395-9402