pLC16-37/WL8      Source of Strain:
pLC16-37 Plasmid Markers/Mutations: araC14
, melA Plasmid Comment:
Use CGSC No. 6592. Hybridizes with Kohara clones 645-646. Chromosomal Markers: recA56
, melA7Strain Comments:
- The plasmid carries the gene melA.
- recA56-- Amino acid substitution Arg 60 Cys
- srlD300::Tn10-- : The insertion was determined by sequencing to be within srlD (F.W.Pons, pers.commun., Oct.2000)
- spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- srlD300::Tn10 was formerly called srlC300::Tn10
- = tetracycline resistant
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation