EDCM382      Source of Strain:
Masters and MerlinSex:
F- Chromosomal Markers: metN558::FLKP2(lacZ,kan,Plac)
, rph-1Strain Comments:
- Δfnr-267-- was reported for MG1655 by pers.commun.from V.Stewart to B.Bachmann,1986, again to CGSC by V.S.2003. Some MG1655 subcultures and derivatives have it .See also Soupene et al. JBact.185:5611(2003).
- rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
- = kanamycin resistant
- Merlin, C., G. Gardiner, S. Durand, M. Masters 2002. The Escherichia coli metD locus encodes an ABC transporter which includes Abc (MetN), YaeE (MetI), and YaeC (MetQ). J.Bacteriol. 184:5513-5517