CGSC Strain#: 8006
Strain Designation: RL425      Source of Strain: R. Lathe
Sex: F- Chromosomal Markers: rpsB2236(ts), relA1, spoT1, metB1, creC510
Strain Comments:

• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• creC510-- The constitutive phenotype is due to an R77P amino acid substitution
• creC510 was formerly called phoM510
• Const = constitutive
• ts = Temperature Sensitive

References:

• Bollen, A., R. Lathe, A. Herzog, D. Denicourt, J.-P. Lecocq, L. Desmarez, R. Lavalle' 1979. A conditionally lethal mutation of Escherichia coli affecting the gene coding for ribosomal protein S2 (rpsB). J.Mol.Biol. 132:219-233
• Moll, I., S. Grill, A. Grundling, U. Blasi 2002. Effects of ribosomal proteins S1, S2 and the DeaD/CsdA DEAD-box helicase on translation of leaderless and canonical mRNAs in Escherichia coli. Mol.Microbiol. 44:1387-1396