CGSC Strain#: 6656
Strain Designation: N3002      Source of Strain: R.G. Lloyd
Sex: F- Chromosomal Markers: λ^-, cysI95::Tn10, IN(rrnD-rrnE)1, rph-1
Strain Comments:

• cysI95::Tn10-- shown by PCR to be inserted after nucleotide 2,886,785 (minute 62.226) in cysI rather than cysC (Nichols et al. 1998).
• IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
• rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
• cysI95::Tn10 was formerly called cysC95::Tn10
• = tetracycline resistant

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