N3002      Source of Strain:
F- Chromosomal Markers: λ-
, rph-1Strain Comments:
- cysI95::Tn10-- shown by PCR to be inserted after nucleotide 2,886,785 (minute 62.226) in cysI rather than cysC (Nichols et al. 1998).
- IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
- rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
- cysI95::Tn10 was formerly called cysC95::Tn10
- = tetracycline resistant