CGSC Strain#: 5881
Strain Designation: ZIP545F      Source of Strain: D. Zipser
Sex: F' Episome/Plasmid:
   Plasmid Markers/Mutations: lacZ545(Am)
Episome/Plasmid: F42-545
   Plasmid Markers/Mutations: lacZ545(Am), lac
Chromosomal Markers: lacZ545(Am), λ^-, trpA49(Am), relA1, rpsL150(strR)
Strain Comments:

• N.B. This strain is a homogenote for lacZ545(Am).
• lacZ545(Am)-- is a UAG mutation.
• trpA49(Am)-- is the amber mutation formerly called trp^-_8am by Brenner.
• rpsL150(strR)-- T to C transition mutation resulting in a K43R amino acid substitution.
• Am = amber(UAG) mutation
• strR = streptomycin resist.
• Am = amber(UAG) mutation
• Am = amber(UAG) mutation

References:

• Morrison, SL, D Zipser 1970. Polypeptide products of nonsense mutations. I. Termination fragments from nonsense mutations in the Z gene of the lac operon of Escherichia coli. J. Mol. Biol. 50(2):359-71.
• Morrison, SL, D Zipser, R Goldschmidt 1971. Polypeptide products of nonsense mutations. II. Minor fragments produced by nonsense mutations in the z gene of the lactose operon of Escherichia coli. J. Mol. Biol. 60(3):485-97.