CGSC Strain#: 8196
Strain Designation: CSH4     
Source of Strain: J.H. Miller
Sex: F-
Chromosomal Markers: lacZ1125,
λ-,
trpA49(Am)
,
relA1,
rpsL150(strR),
spoT1Strain Comments: - lacZ1125-- is a nonsense mutation in interval 6 of the lacZ gene. It may be the NG125 amber mutation of Newton et al, 1965 or one of the other interv, 6 nonsense mutations of Zipser et al.1970.
- trpA49(Am)-- is the amber mutation formerly called trp-8am by Brenner.
- rpsL150(strR)-- T to C transition mutation resulting in a K43R amino acid substitution.
- spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- Am = amber(UAG) mutation
- strR = streptomycin resist.
References: - Newton, W.A., J.R. Beckwith, D. Zipser, S. Brenner 1965. Nonsense mutants and polarity in the lac operon of Escherichia coli. J.Mol.Biol. 14:290-296
- Zipser, D, S Zabell, J Rothman, T Grodzicker, M Wenk 1970. Fine structure of the gradient of polarity in the z gene of the lac operon of Escherichia coli. J. Mol. Biol. 49(1):251-4.