CGSC Strain#: 8194
Strain Designation: JT31      Source of Strain: J.M. Wood
Sex: F- Chromosomal Markers: lacZ1125, λ^-, putA1::Tn5, trpA49(Am), relA1, rpsL150(strR), spoT1
Strain Comments:

• Cannot utilize proline as C or N source. More sensitive to L-azetidine-w-carboxylate and 3,4-dehydroproline than parent, CSH4
• lacZ1125-- is a nonsense mutation in interval 6 of the lacZ gene. It may be the NG125 amber mutation of Newton et al, 1965 or one of the other interv, 6 nonsense mutations of Zipser et al.1970.
• putA1::Tn5-- results in white colonies on proline-supplemented TTC medium (Bochner and Savageau, Appl.Environ.Microbiol.33:434)
• trpA49(Am)-- is the amber mutation formerly called trp^-_8am by Brenner.
• rpsL150(strR)-- T to C transition mutation resulting in a K43R amino acid substitution.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• = kanamycin resistant
• Am = amber(UAG) mutation
• strR = streptomycin resist.

References:

• Wood, J.M. 1981. Genetics of L-proline utilization in Escherichia coli. J.Bacteriol. 146:895-901