CGSC Strain#: 8195
Strain Designation: WG350     
Source of Strain: J.M. Wood
Sex: F-
Chromosomal Markers: lacZ1125,
λ-,
Δ(putA-putP)101,
trpA49(Am)
,
ΔproU-600,
relA1,
rpsL150(strR),
spoT1,
Δ(proP-melB)212Strain Comments: - lacZ1125-- is a nonsense mutation in interval 6 of the lacZ gene. It may be the NG125 amber mutation of Newton et al, 1965 or one of the other interv, 6 nonsense mutations of Zipser et al.1970.
- trpA49(Am)-- is the amber mutation formerly called trp-8am by Brenner.
- ΔproU-600-- Registered under E.Bremer as proU600. Is this the same as the J.W.Wood deletion DE(proU)600?
- rpsL150(strR)-- T to C transition mutation resulting in a K43R amino acid substitution.
- spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- ΔproU-600 was formerly called DE(proV-X)601
- Am = amber(UAG) mutation
- strR = streptomycin resist.
References: - Culham, D.E., B. Lasby, A.G. Marangoni, J.L. Milner, B.A. Steer, R.W. van Nues, J.M. Wood 1993. Isolation and sequencing of Escherichia coli gene proP reveals unusual structural features of the osmoregulatory proline/betaine transporter, ProP. J.Mol.Bi