CGSC Strain#: 8195
Strain Designation: WG350      Source of Strain: J.M. Wood
Sex: F- Chromosomal Markers: lacZ1125, λ^-, Δ(putA-putP)101, trpA49(Am), ΔproU-600, relA1, rpsL150(strR), spoT1, Δ(proP-melB)212
Strain Comments:

• lacZ1125-- is a nonsense mutation in interval 6 of the lacZ gene. It may be the NG125 amber mutation of Newton et al, 1965 or one of the other interv, 6 nonsense mutations of Zipser et al.1970.
• trpA49(Am)-- is the amber mutation formerly called trp^-_8am by Brenner.
• ΔproU-600-- Registered under E.Bremer as proU600. Is this the same as the J.W.Wood deletion DE(proU)600?
• rpsL150(strR)-- T to C transition mutation resulting in a K43R amino acid substitution.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• ΔproU-600 was formerly called DE(proV-X)601
• Am = amber(UAG) mutation
• strR = streptomycin resist.

References:

• Culham, D.E., B. Lasby, A.G. Marangoni, J.L. Milner, B.A. Steer, R.W. van Nues, J.M. Wood 1993. Isolation and sequencing of Escherichia coli gene proP reveals unusual structural features of the osmoregulatory proline/betaine transporter, ProP. J.Mol.Bi