CGSC Strain#: 6994
Strain Designation: BSV23      Source of Strain: P.M. Rabinovich
Sex: F- Chromosomal Markers: glnX44(AS), λ^-, mcrA0, ribC23::Tn5, rfbC1, endA1, spoT1, thiE1, mcrB9999, hsdR29
Strain Comments:

• This strain is thought to carry mutations that render it permeable to riboflavin.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• endA1-- G to A transition mutation resulting in E208K amino acid substitution.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• = good miniprep dsDNA quality
• = kanamycin resistant
• AS = amber(UAG) suppressor

References:

• Bandrin, SV, PM Rabinovich, AI Stepanov 1983. [3 linkage groups of the genes of riboflavin biosynthesis in Escherichia coli]. Genetika 19(9):1419-25.