RL254      Source of Strain:
F- Chromosomal Markers: [araD139]B/r
, deoC1Strain Comments:
- [araD139]B/r-- The thr-leu region was transduced from an E. coli B/r strain (SB3118) in early steps of strain derivation; ara or in some strains ara-leu or thr-leu are from B.
- e14--- : Loss of the e14 element makes the strain McrA-.
- flhD5301-- flagellar defect shown in Komeda and Iino
- Δ(fruK-yeiR)725(fruA25)-- : this fruA25 mutation deletes 29 codons of fruK, fruB and fruBKAp promoter to orf yeiR Peters et al. J.Bact.185:2017.
- rpsL150(strR)-- T to C transition mutation resulting in a K43R amino acid substitution.
- Δ(fruK-yeiR)725(fruA25) was formerly called fruA25
- ::IS1 = insertion of IS1
- del-ins = deletion-insertion
- fruA25 = former designation
- strR = streptomycin resist.
- Lovingshimer, M.R., D.A. Siegele, G.D. Reinhart 2006. Construction of an inducible, pfkA and pfkB deficient strain of Escherichia coli for the expression and purification of phosphofructokinase from bacterial sources. Protein Expr.Purif. 46:475-482