CGSC Strain#: 8262
Strain Designation: JKD42/pKD123     
Source of Strain: J. Lutkenhaus
Sex: F-
Episome/Plasmid: pKD123
Plasmid Markers/Mutations: cat,
oripEL3(ts)
,
ftsN Chromosomal Markers: λ-,
recA56,
srlD300::Tn10,
IN(rrnD-rrnE)1,
rph-1,
ftsN110::kanStrain Comments: - recA56-- Amino acid substitution Arg 60 Cys
- srlD300::Tn10-- : The insertion was determined by sequencing to be within srlD (F.W.Pons, pers.commun., Oct.2000)
- IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
- rph-1-- is a 1 bp deletion that results in frameshift over last 15 codons and has polar effect on pyrE leading to suboptimal pyrimidine levels on minimal medium.(Jensen 1993 JBact.175:3401)
- srlD300::Tn10 was formerly called srlC300::Tn10
- = tetracycline resistant
- = kanamycin resistant
- ts = Temperature Sensitive
References: - Dai, K., Y. Xu, J. Lutkenhaus 1993. Cloning and characterization of ftsN, an essential cell division gene in Escherichia coli isolated as a multicopy suppressor of ftsA12(Ts). J.Bacteriol. 175:3790-3797