CGSC Strain#: 6114
Strain Designation: JW164      Source of Strain: J.A. Wechsler
Sex: F- Chromosomal Markers: lacZ53(Am), λ^-, thyA36, IN(rrnD-rrnE)1, rpsL151(strR), polA1(Am), rha-5, malB45, deoC2
Strain Comments:

• lacZ53(Am)-- is the Paris lac^-_{Y14 amber}.
• thyA36-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• IN(rrnD-rrnE)1-- Inverts the region between rrnD (73.74 min) and rrnE (90.66 min)
• polA1(Am)-- normal levels exonuc.;premature term.of polymerase <1% WT level. MMS^S,UV^S, viable at all temps. Grindley MGG143
• Am = amber(UAG) mutation
• strR = streptomycin resist.

References:

• Lark, KG, JA Wechsler 1975. DNA replication in dnaB mutants of Escherichia coli: gene product interaction and synthesis of 4 S pieces. J. Mol. Biol. 92(1):145-63.