CGSC Strain#: 2500
Strain Designation: AB2500     
Source of Strain: P. Howard-Flanders
Sex: F-
Chromosomal Markers: thr-1,
araC14,
leuB6(Am)
,
Δ(gpt-proA)62,
lacY1,
tsx-33,
qsr'-0,
glnX44(AS)
,
galK2(Oc)
,
λ-,
hisG4(Oc)
,
thyA15,
rpsL31(strR)
,
xylA5,
mtl-1,
argE3(Oc)
,
thiE1,
uvrA6,
deoB12Strain Comments: - leuB6(Am)-- Suppressed by serU suppressor (supH)
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- thyA15-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
- Δ(gpt-proA)62 was formerly called proA2
- galK2(Oc) was formerly called gal2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
- strR = streptomycin resist.
References: - Howard-Flanders, P., R.P. Boyce, L. Theriot 1966. Three loci in Escherichia coli K-12 that control the excision of pyrimidine dimers and certain other mutagen products from DNA. Genetics 53:1119-1136
- Rupp, WD, P Howard-Flanders 1968. Discontinuities in the DNA synthesized in an excision-defective strain of Escherichia coli following ultraviolet irradiation. J. Mol. Biol. 31(2):291-304.