CGSC Strain#: 1515
Strain Designation: 13-6     
Source of Strain: M. Schwartz
Other Designations: AB1515
Other Designations: χ214
Other Designations: chi214
Sex: F-
Chromosomal Markers: leuB6(Am)
,
secA206(aziR)
,
fhuA23,
lacY1,
proC14,
tsx-67,
purE42,
glnX44(AS)
,
λ-,
trpE38,
rpsL109(strR)
,
xylA5,
mtl-1,
thiE1Strain Comments: - The lac marker was mistakenly called lacZ in Schwartz 1963 Genetics 38:1357.
- leuB6(Am)-- Suppressed by serU suppressor (supH)
- purE42-- :Strains with this marker are derivatives of Χ148 and may carry malA38LAMR and a suppressor of the Mal- phenotype, sup-78,near his.
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- aziR = azide resistance
- strR = streptomycin resist.
References: - SCHWARTZ, NM 1964. SUPPRESSION OF A LAC O-O MUTATION IN ESCHERICHIA COLI. J. Bacteriol. 88:996-1001.
- Elish, M.E., J.R. Pierce, C.F. Earhart 1988. Biochemical analysis of spontaneous frpA mutants of Escherichia coli. J.Gen.Microbiol. 134:1355-1364