CGSC Strain#: 1623
Strain Designation: AB1623      Source of Strain: H.L. Kornberg
Sex: F- Chromosomal Markers: araC14, lacY1, tsx-57, glnX44(AS), gltA5, galK2(Oc), Rac-0, rfbC1, rpsL20(strR), xylA5, mtl-1, lldD1, thiE1, tfr-5
Strain Comments:

• For discussion of membrane defect in parent strain AB1621 see: Schnaitman 1974 J. Bacteriol 118:454.
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

References:

• Ashworth, J.M., H.L. Kornberg, D.L. Nothmann 1965. Location of the structural gene for citrate synthase on the chromosome of Escherichia coli K12. J.Mol.Biol. 11:654-657