EM120      Source of Strain:
F- Chromosomal Markers: araC14
, tfr-5Strain Comments:
- This strain must be grown in the presence of 200 ug/ml stretomycin in order to prevent selection of faster-growing revertants of the streptomy cin-suppressible lethal mutation gltM4.
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- gltM4-- affects level of glutamyl tRNA synthetase??
- ilv-275-- The ilv-275 mutation renders this strain conditionally streptomycon-dependent.
- galK2(Oc) was formerly called gal2
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called glnV44
- rfbC1 was formerly called rfbD1
- thiE1 was formerly called thi-1
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
- strR = streptomycin resist.
- Murgola, E., E.A. Adelberg 1970. Mutants of Escherichia coli K-12 with an altered glutamyl-transfer ribonucleic acid. J.Bacteriol. 103:178-183