CGSC Strain#: 4176
Strain Designation: EM102      Source of Strain: E. Murgola
Sex: F- Chromosomal Markers: araC14, lacY1, tsx-57, glnV44(AS), galK2(Oc), rfbC1, mgl-51, rpsL20(strR), xylA5, mtl-1, gltE3, thiE1, ilv-275, tfr-5
Strain Comments:

• This strain must be grown in the presence of 200 ug/ml streptomycin in order to prevent selection of faster-growing revertants of the streptomycin-suppressible lethal mutation gltE3.
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• ilv-275-- The ilv-275 mutation renders this strain conditionally streptomycon-dependent.
• galK2(Oc) was formerly called gal₂
• glnV44(AS) was formerly called supE44
• glnV44(AS) was formerly called su⁺_II
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

References:

• Murgola, E., E.A. Adelberg 1970. Mutants of Escherichia coli K-12 with an altered glutamyl-transfer ribonucleic acid. J.Bacteriol. 103:178-183