CGSC Strain#: 4246
Strain Designation: KL722     
Source of Strain: M.C. Cappello
Sex: F'
Episome/Plasmid: F131
Plasmid Markers/Mutations: serV43(AS)
Chromosomal Markers: leuB6(Am)
,
fhuA2,
lacY1,
tsx-1,
glnV44(AS)
,
gal-6,
λ-,
hisG1(Fs)
,
recA1,
argG6,
rpsL104,
malT1(λ
R)
,
xyl-7,
mtlA2,
metB1Strain Comments: - This strain must be maintained on medium containing arg, leu, met and lacking his.
- leuB6(Am)-- Suppressed by serU suppressor (supH)
- recA1-- : Missense mutation, altered isoelectric point. Sequenced: G to A for Nuc. 720 (Gly 160 Asp).
- glnV44(AS) was formerly called supE44
- glnV44(AS) was formerly called su+II
- xyl-7 was formerly called xylA7
- = strep-resistant
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- Fs = Frameshift mutation
- LamR = Lambda-resistant
- AS = amber(UAG) suppressor
References: - Low, KB 1972. Escherichia coli K-12 F-prime factors, old and new. 36(4):587-607.