CGSC Strain#: 6371
Strain Designation: HF4735      Source of Strain: P. Howard-Flanders
Sex: F- Chromosomal Markers: lac-61, glnV44(AS), λ^-, uvrB5, thyA48, endA1, deo-27, φX^s
Strain Comments:

• The rfa region of this strain comes from E. coli C.
• thyA48-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• endA1-- G to A transition mutation resulting in E208K amino acid substitution.
• deo-27-- deoB or deoC.
• φX^s-- : φX sensitivity is derived from E. coli C
• glnV44(AS) was formerly called supE44
• glnV44(AS) was formerly called su⁺_II
• = good miniprep dsDNA quality
• AS = amber(UAG) suppressor

References: