CGSC Strain#: 6414
Strain Designation: SS320      Source of Strain: S.A. Short
Sex: F- Chromosomal Markers: secA209(aziR), pro-48, lacZ118(Oc), lacI22, λ^-, trpA9705(Am), his-85(Am), gyrA19(NalR), rpsL171(strR), metE70, trpR55
Strain Comments:

• This strain is KL320 cured of a 60MD plasmid which conferred immunity to P1.
• pro-48-- is probably in proB^-.
• lacZ118(Oc)-- is the lacZ allele originating in Paris strain Hfr 3000 U118. It is an ochre mutation.
• his-85(Am)-- is an amber mutation. It is suppressed by supD32.
• trpR55-- is trpR2 of Yanofsky. It is a frameshift mutation. Trp levels are indistinguishable from those in trpR deletions or amber mutations (no functional repressor).
• his-85(Am) was formerly called his-29
• lacI22 was formerly called lac_i3^-
• lacI22 was formerly called lacI3
• secA209(aziR) was formerly called azi-9
• Am = amber(UAG) mutation
• aziR = azide resistance
• NalR = Nalidix.acidResist.
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

References:

• Birge, EA, KB Low 1974. Detection of transcribable recombination products following conjugation in rec+, reCB- and recC-strains of Escherichia coli K12. J. Mol. Biol. 83(4):447-57.