CGSC Strain#: 5948
Strain Designation: ZIP514      Source of Strain: D. Zipser
Sex: F- Chromosomal Markers: Δ(codB-lacI)3, tsx-93, λ^-, trpA49(Am), relA1, rpsL150(strR), spoT1
Strain Comments:

• Δ(codB-lacI)3-- is a deletion of the entire lac operon, from the strain Hfr 3000 X74 of Jacob. Refs. on extent of deletion: Sung et al.1987,J.Bact.169:2639; Gerdes et al.2001
• Δ(codB-lacI)3-- Shown by Ferrandez et al. 1997 to extend to mhpD
• Δ(codB-lacI)3-- Shown by Durfee et al. to extend from codon 292 of yahG through mhpE
• trpA49(Am)-- is the amber mutation formerly called trp^-_8am by Brenner.
• rpsL150(strR)-- T to C transition mutation resulting in a K43R amino acid substitution.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• Δ(codB-lacI)3 was formerly called Δlac74
• Δ(codB-lacI)3 was formerly called Δ(lac)3
• Δ(codB-lacI)3 was formerly called Δ(yahG-mhpE)
• Δ(codB-lacI)3 was formerly called X74
• Δ(codB-lacI)3 was formerly called DE(lac)6
• Am = amber(UAG) mutation
• strR = streptomycin resist.

References:

• Zubay, G, DE Morse, WJ Schrenk, JH Miller 1972. Detection and isolation of the repressor protein for the tryptophan operon of Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 69(5):1100-3.