CGSC Strain#: 5951
Strain Designation: QE35      Source of Strain: M.G. Sunshine
Sex: Hfr    Point of Origin#: 1   Map Position: 96.80   Direction of Marker Transfer: Clockwise
Chromosomal Markers: λ^-, e14-, Δ(attP2H-rfb)87, relA1, spoT1, thiE1
Strain Comments:

• This strain is a P2 eductant.
• e14--- : Loss of the e14 element makes the strain McrA^-.
• Δ(attP2H-rfb)87-- : Originally described as Δ attP2H-rfb87, later as extending through mgl. The deletion length and loss of metG which it would signify favor the rfb endpoint.
• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• thiE1 was formerly called thi-1

References:

• Sunshine, M., B. Kelly 1971. Extent of host deletions associated with bacteriophage P2-mediated eduction. J.Bacteriol. 108:695-704
• Clark, A.F., R.W. Hogg 1981. High-affinity arabinose transport mutants of Escherichia coli: isolation and gene location. J.Bacteriol. 147:920-924