CGSC Strain#: 5990
Strain Designation: TKL46     
Source of Strain: A. Rorsch
Other Designations: H843
Other Designations: PC1242
Sex: F-
Chromosomal Markers: thr-1,
leuB6(Am)
,
murF2,
fhuA21,
codA1,
lacY1,
tsx-95,
glnX44(AS)
,
λ-,
pyrF101,
his-108,
thyA6,
argG66,
ilvA634,
thiE1,
deoC1Strain Comments: - leuB6(Am)-- Suppressed by serU suppressor (supH)
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- thyA6-- : Even with Luria or other complete media, supplement with thymine.
- thyA6-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
References: - Lugtenberg, EJ, A v Schijndel-van Dam 1972. Temperature-sensitive mutants of Escherichia coli K-12 with low activities of the L-alanine adding enzyme and the D-alanyl-D-alanine adding enzyme. J. Bacteriol. 110(1):35-40.