CGSC Strain#: 5103
Strain Designation: HF4733      Source of Strain: P. Howard-Flanders
Sex: F- Chromosomal Markers: lac-61, gal-44, λ^-, thyA48, endA1, deo-43 or deo-29, φX^s
Strain Comments:

• thyA48-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
• endA1-- G to A transition mutation resulting in E208K amino acid substitution.
• φX^s-- : φX sensitivity is derived from E. coli C
• = good miniprep dsDNA quality

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