HF4733      Source of Strain:
F- Chromosomal Markers: lac-61
, deo-43 or deo-29
, φXsStrain Comments:
- thyA48-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
- endA1-- G to A transition mutation resulting in E208K amino acid substitution.
- φXs-- : φX sensitivity is derived from E. coli C
- = good miniprep dsDNA quality