CGSC Strain#: 6033
Strain Designation: PAM163      Source of Strain: B.F. Johnson
Sex: F- Chromosomal Markers: thr-1, araC14, leuB6(Am), Δ(gpt-proA)62, lacY1, tsx-33, lon-22, glnX44(AS), galK2(Oc), λ^-, sulA27, Rac-0, hisG4(Oc), rfbC1, rpsL31(strR), kdgK51, xylA5, mtl-1, metF24, thiE1
Strain Comments:

• leuB6(Am)-- Suppressed by serU suppressor (supH)
• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
• Δ(gpt-proA)62 was formerly called proA2
• galK2(Oc) was formerly called gal₂
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• rfbC1 was formerly called rfbD1
• thiE1 was formerly called thi-1
• Am = amber(UAG) mutation
• AS = amber(UAG) suppressor
• Oc = ochre(UAA) mutation
• strR = streptomycin resist.

References:

• Donch, J., J. Greenberg 1968. Genetic analysis of lon mutants of strain K-12 of Escherichia coli. Mol.Gen.Genet. 103:105-115
• Johnson, B.F. 1977. Fine structure mapping and properties of mutations suppressing the lon mutation in Escherichia coli K-12 and B strains. Genet.Res. 30:273-286