Access To and Information About All Query Forms.
- Which Query form should I use?
- Strain Query
- Mutation Query
- Gene/Plasmid/Phage/Chrom.Sites Query
- Gene Product Query
- Reference Query
- Query Help
Links and lists of commonly requested and searched for strains
- Browsable list of Keio Knockout Strains
- List of commonly requested strains auxotrophic for amino acids
- Strain kits for using the Wanner Lambda Red method of gene disruption
TO REQUEST STRAINS or additional information, email us at CGSC@yale.edu or see our other contact information. When requesting strains please include the shipping address and the name of the Primary Investigator of the laboratory. A description of the charges for strains can be found here.
Note: The CGSC Collection contains only non-pathogenic BSL-1 laboratory strains, primarily genetic derivatives of Escherichia coli K-12, the laboratory strain widely used in genetic and molecular studies. For details, see About CGSC in the navigation bar on the left.
NEW: Single-stranded DNA size markers for gel electrophoresis. ss100 DNA Ladder gives bands at 100-nucleotide increments with sizes ranging from 100 to greater than 1000 nucleotides.
The CGSC Database of E. coli genetic information includes genotypes and reference information for the strains in the CGSC collection, the names, synonyms, properties, and map position for genes, gene product information, and information on specific mutations and references to primary literature. The public version of the database includes this information and can be queried directly via this CGSC DB WebServer. For help, use the help links located above and on each query form, or contact us directly.