ss200 DNA Ladder
ss200 DNA Ladder can be used as size markers for single-stranded DNAs or RNAs. Researchers working with single-stranded DNA bacteriophages, DNA origami or DNA nanotechnology samples, rRNAs, mRNAs, or lincRNAs may find these markers to be particularly useful.
ss200 DNA Ladder is prepared by the laboratory of Ronald Breaker at Yale University by employing self-hydrolyzing deoxyribozymes to process multimeric DNA amplification products (1). The samples are produced as a core facility service and the fee for the samples offsets the cost of preparation and storage.
(100 μL each)
(Shipping not included)
To Request Samples: Requests are currently handled via email. Send request to: firstname.lastname@example.org
ss200 DNA Ladder is a single-stranded DNA marker preparation (100 microliters) containing DNA molecules of 200-nucleotide increments ranging from 200 nucleotides to more than 3000 nucleotides. The total DNA concentration is approximately 0.01 micrograms per microliter in a solution containing 50 mM HEPES (pH 7.0 at 23°C), 100 mM NaCl, 2 mM ZnCl2, and 2.5 mM EDTA.
Recommended uses: Combine approximately 5 to 10 microliters of ss200 DNA Ladder solution with your loading buffer to load in each gel lane and stain the gel with a DNA-binding dye to visualize (e.g. Fig. 1). Alternatively, the DNA marker can be 5′ radiolabeled using polynucleotide kinase. Note that the DNAs carry a 5′ phosphate group, which would need to be removed prior to labeling by using polynucleotide kinase. Intended for use only on polyacrylamide gels (see Fig. 1).
Storage: Extended storage is recommended at -20°C. Avoid long-term storage at low pH and avoid exposure to DNases.
Reference: Gu, H. and Breaker, R. R. (2013) Production of single-stranded DNAs by self-cleavage of rolling-circle amplification products, BioTechniques 54:337-343.
Fig. 1. Representative ss200 DNA ladder preparation separated by denaturing 8% PAGE. 5 μL of ss200 DNA ladder plus a loading buffer was loaded on an 8-mm-wide, 0.75 mm-thick well. SYBR Gold Nucleic Acid Gel Stain was used for detection. Distortion of the 1000-nt band is due to loading dye interference. Band intensities may vary for different lots.