Mutation arcA726(del)::kan
- Name: arcA726(del)::kan
- Type: Insertion
- Mutation of Gene: arcA
- Approx. Map Location: 99.96
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were GCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAATTCCGGGGATCCGTCGACC and CTTTTGTACTTCCTGTTTCGATTTAGTTGGCAATTTAGGTAGCAAACATGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW4364-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514, ΔarcA726::kan