Mutation ascB720(del)::kan
- Name: ascB720(del)::kan
- Type: Insertion
- Mutation of Gene: ascB
- Approx. Map Location: 61.20
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were GTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGATTCCGGGGATCCGTCGACC and GGCGTTCACGCCGCATCCGGCACTGTTACCTACTCTAAATCTTCCCCATTTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW2686-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, ΔascB720::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514