Mutation carB745(del)::kan
- Name: carB745(del)::kan
- Type: Insertion
- Mutation of Gene: carB
- Approx. Map Location: 0.66
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were ATTGAGCAGTACCGTAAAACCGCTAAGTAATCAGGAGTAAAAGAGCCATGATTCCGGGGATCCGTCGACC and GGATGAAAAATATCTGCCATGACACGCTATTATTTGATCTGTGCGTGCATTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW0031-1 7 F-, ΔcarB745::kan, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514