Mutation ccmH747(del)::kan
- Name: ccmH747(del)::kan
- Type: Insertion
- Mutation of Gene: ccmH
- Approx. Map Location: 49.35
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were GCCCGATGGCATCACCATCGGGCCTCTTTTTATTTACTCTCCTGCGGCGAATTCCGGGGATCCGTCGACC and GAAGAGATCAAGCCGCTGTGGGAGAAATACAGTAAGGAGGCCGCACAATGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW2182-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, ΔccmH747::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514