Mutation chaC729(del)::kan
- Name: chaC729(del)::kan
- Type: Insertion
- Mutation of Gene: chaC
- Approx. Map Location: 27.41
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were AGCAGTAAAAATGTCCGGAAGACACCAAAAAGTTGTCGCAGGGAAGTATGATTCCGGGGATCCGTCGACC and TCTGACGCCCCACTATGCGGGAAGTTTACTCAGGCGAATCCCGGACGTAATGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW1209-2 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, ΔchaC729::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514