Mutation dnaT759(del)::kan
- Name: dnaT759(del)::kan
- Type: Insertion
- Mutation of Gene: dnaT
- Approx. Map Location: 99.12
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were TTGCATCAGGTCGCCAACGTTTTTCATCGTTACCCTCTGAATCCTGGTGGATTCCGGGGATCCGTCGACC and AAAAGTTATCTCCCTTCTCGTTCATCGTTCCATATTTGAGAAACAGTATGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW4326-2 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514, ΔdnaT759::kan