Mutation eda-775(del)::kan
- Name: eda-775(del)::kan
- Type: Insertion
- Mutation of Gene: eda
- Approx. Map Location: 41.61
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were GCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACATTCCGGGGATCCGTCGACC and TCACTTTTTAAGACGACAAATTTGTAATCAGGCGAGAGAAAACTCTGATGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW1839-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, Δeda-775::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514