Mutation gcl-790(del)::kan
- Name: gcl-790(del)::kan
- Type: Insertion
- Mutation of Gene: gcl
- Approx. Map Location: 11.49
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were AATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGATTCCGGGGATCCGTCGACC and GCAGAGAAACGTAACATTATTTATCTCCCTTATTCATAGTGCATGAAGCATGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW0495-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), Δgcl-790::kan, λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514