Mutation gnd-727(del)::kan
- Name: gnd-727(del)::kan
- Type: Insertion
- Mutation of Gene: gnd
- Approx. Map Location: 45.22
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were GCCTCAATTTTATTGTTGGTTAAATCAGATTAATCCAGCCATTCGGTATGATTCCGGGGATCCGTCGACC and AGGCCGCGAGCATTCAGCGCGGTGATCACACCTGACAGGAGTATGTAATGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW2011-2 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, Δgnd-727::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514